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Hozier博士在佛羅里達(dá)州立大學(xué)分子生物物理研究所進(jìn)行染色體復(fù)制研究生工作，并在Tübingen的馬克斯·普朗克研究所進(jìn)行染色體分子組織研究工作，德國(guó)。他曾在明尼蘇達(dá)大學(xué)醫(yī)學(xué)院，佛羅里達(dá)州立大學(xué)佛羅里達(dá)州立大學(xué)（分子生物物理研究所）和新墨西哥州立大學(xué)醫(yī)學(xué)院任教。他于1984年創(chuàng)立了AGL。他的研究興趣集中在理解染色體畸變和染色體分子組織。他的臨床興趣包括使用DNA探針的高分辨率核型分析和熒光原位雜交，現(xiàn)在通常用于臨床工作來(lái)定義癌癥中的特定染色體重排和發(fā)育異常。他是美國(guó)醫(yī)學(xué)遺傳學(xué)院的研究員，并獲得美國(guó)醫(yī)學(xué)遺傳學(xué)委員會(huì)臨床細(xì)胞遺傳學(xué)認(rèn)證。曾任多所大學(xué)和商業(yè)機(jī)構(gòu)的臨床實(shí)驗(yàn)室主任。

Bryan K Hall，MS，CG（ASCP）^CM 技術(shù)總監(jiān) Bryan從佛羅里達(dá)理工學(xué)院獲得細(xì)胞和分子生物學(xué)碩士學(xué)位，對(duì)致突變性測(cè)定中使用的組織培養(yǎng)系進(jìn)行染色體分析。他在人類，小鼠，大鼠，猴子等多種物種的細(xì)胞遺傳學(xué)分析和染色體鑒定方面擁有30年經(jīng)驗(yàn)。布萊恩在約翰霍普金斯大學(xué)醫(yī)學(xué)院和密歇根大學(xué)兒科遺傳學(xué)系擔(dān)任過(guò)學(xué)術(shù)職務(wù)，并獲得了位于新墨西哥州阿爾伯克基市TriCore參考實(shí)驗(yàn)室的臨床細(xì)胞遺傳學(xué)經(jīng)驗(yàn)，負(fù)責(zé)細(xì)胞遺傳學(xué)實(shí)驗(yàn)室的技術(shù)和監(jiān)管工作。除了染色體鑒定，Bryan精通染色體畸變分析，致突變性分析和熒光原位雜交（FISH），包括探針生產(chǎn)。他已經(jīng)在人類臨床組織，建立的細(xì)胞系，體細(xì)胞雜交體和實(shí)驗(yàn)室小鼠的組織培養(yǎng)樣品上工作多年。他的分子生物學(xué)技術(shù)包括核酸純化，PCR，大插入克隆繁殖和操縱以及基因作圖。Bryan曾在AGL工作了8年，目前擔(dān)任技術(shù)總監(jiān)，他是Cell Line Characterization Studies的研究總監(jiān)。

Hybloc™ competitor DNA is the repetitive sequence fraction of genomic DNA. Hybloc™ competitor DNA may be effectively substituted for any other competitor DNA (i.e., COT-1 DNA®) for fluorescence in situ hybridization (FISH), Southern blots, microarray technology, or any other protocol requiring competition to prevent repetitive DNA from binding to the target. Hybridization with Hybloc™ competitor DNA shows a marked increase in signal intensity and a significant decrease in the amount of background noise associated with repetitive DNA. Hybloc™ competitor DNA is available for the following species: Mouse, Rat, Human, Bovine, Ovine, Porcine, Chicken, and Canine.

Product: Ovine Hybloc DNA

Catalog #: OHB

Concentration: 1 mg/ml (A260)

Source: Male Ovine Tissues

Storage: -20°C

Description:

Ovine Hybloc DNA is the repetitive sequence fraction of genomic DNA obtained from male ovine tissues by extracting, shearing, denaturing, and reannealing DNA under conditions that enrich for repetitive sequences.

Ovine Hybloc DNA may be effectively substituted for any other competitor DNA (e.g., COT-1 DNA , Salmon Sperm DNA, Genomic DNA, etc.) to suppress cross-hybridization to ovine repetitive sequences for in situ hybridization, Southern blots, microarray technology, or any other protocol requiring competition to block repetitive DNA from binding to the target. Hybridization with Hybloc DNA shows a marked increase in signal intensity and a significant decrease in the amount of background noise associated with repetitive DNA.

How Supplied:

Ovine Hybloc DNAs are supplied in various quantities at 1mg/ml in 10 mM Tris-HCl (pH 7.4), 1 mM EDTA.

Quality Control Assay:

Determination of concentration from the A260 and determination of the A260/A280 ratio (1.8 to 2.0); determination of molecular size: >80% of Ovine Hybloc DNA will run as a broad band between the 50 bp and 600 bp size range.

References:

Landegent JE, Jansen in de Wal N, Dirks RW, Baao F, van der Ploeg M. Use of whole cosmid cloned genomic sequences for chromosomal localization by non-radioactive in situ hybridization. Human Genetics 1987 Dec;77(4):366-70.

Lengauer C, Riethman H, Cremer T. Painting of human chromosomes with probes generated from hybrid cell lines by PCR with Alu and L1 primers. Human Genetics 1990 Nov;86(1):1-6.

Lichter P, Cremer T, Borden J, Manuelidis L, Ward DC. Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Human Genetics 1988 Nov;80(3):224-34.

Lichter P, Tang CJ, Call K, Hermanson G, Evans GA, Housman D, Ward DC. High resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 1990 Jan5;247(4938):64-9.

Scalzi JM, & Hozier, JC. Comparative genome mapping: mouse and rat homologies revealed by fluorescence in situ hybridization. Genomics 1998 Jan1;47(1):44-51.

COT-1 DNA is a registered trademark of Invitrogen Corporation.

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